• Samples of three different types of analgesics
• Glass pipet(prepared in class)
• Glacial acetic acid
• 1,2-dichloromethane
• 95% ethanol
• acetone
• mixed "hexanes"
• ethyl acetate
• acetic acid
• three thin layer plates impregnated with silica gel
• UV lamp
• 3-500mL beakers(used as developers)

Procedures

• pulverize sample to a fine powder with the back  of laboratory spoon
• prepare a glass pipet  by warming the pipet and stretch when it is soft,  break  the pipet to create  to small tipped pipets.
• took a small amount of the powdered  analgesic and place it in a beaker 
• mixed powered analgesic (aleve) with a small amount of 95% ethanol and swabbed a small amount on the tip of the glass pipet
• placed swabbed pipet at the bottom of three large silica plates and dotted with a pencil to indicate where it was placed. 
• swabbed two other analgesics (aspirin and ibuprofen) and caffeine with the glass pipet and dotted it on the silica plate
• Also dotted with a pencil to indicate where products had been placed
• Prepared three following mixtures:

        a) 1:12 (acetic acid: 1,2-dichloroethane) in mL

       b) 25:1 (ethanol: acetyl acetone) in mL         

       c) 5:1:1:2 (1,2-dichloroethane: acetone: ethanol: mixed hexanes) in mL

• Placed silica plates in each of the mixtures and allowed them to rise until 1/8 in. of tip
• Allowed to dry and placed all three silica plates under ultraviolet (UV) light and circled where the color had moved.
• Placed all three silica plates in an iodine bath and let sit for 10 minutes.
• After soaking in iodine bath we then circled the stains where each color (analgesic) had moved.

Observations:

• Take a look at connecting page for our Rf values
• All three mixtures were clear (like water) and gave off a strong aroma
• After iodine bath our lead was the only analgesic bound by the iodine.
• Caffeine dot hardly moved at all.